Progressive and Modified Progressive Staining

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Progressive and Modified Progressive Staining

Progressive staining occurs when the hematoxylin is added to the tissue without being followed by a differentiator to remove excess dye. Modified progressive staining occurs when a mild differentiator is used not to remove excess stain from the nuclei, but rather removes background staining. So, the key to whether a stain is progressive is whether the nuclear stain is altered, not whether there is a differentiator present.