Initial Observations of Antibody Panel

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Antibody Detection and Identification (online CE course)
Initial Observations of Antibody Panel

In this slide, we'll explore examples that demonstrate how our prior discussion from the last page can aid in characterizing the reactivity of a suspected antibody.
  • Look at the phase in which reactions are occurring. Reactions at immediate spin (IS) usually are not clinically significant. Reactions at AHG are clinically significant.
  • Look at the strengths of reactions. Varying strengths of reactions could indicate dosage. In the example below, the heterozygous (Jka Jkb) cells react weaker with a 2+ reaction. The homozygous cells (Jkb Jkb) react stronger with a 3+ reaction.
  • Varying strengths may also mean more than one antibody is present.
Rh-HrKellDuffyKiddLewisMNSsP1Lutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+02+NT
2++00++++0+0+++0+0+0++++0+03+NT
3+0++000+0+0+0+0+0++00++0+03+NT
4+0+0+00+0+0+00+00++++0+0+002+
50++0+00+0+0++++++00+0+00+02+NT
600+++0++0+0+0+++0+++++00+02+NT
700+0+00+0+0++00++0+00++0+03+NT
800+0+00+0+0+++0+0+++++00+03+NT
900+0+00+0+0++++00+0++0+0+002+
10+0+0+0++0+0+0+0+0+0++0+0+03+NT
AC002+
IS = immediate spin; AHG = antihuman globulin; CC = check cells; NT = not tested; AC = autocontrol