Enzyme Cofactors, dNTPs, and Salts/Buffers

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course . Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about (online CE course)
Enzyme Cofactors, dNTPs, and Salts/Buffers

It is important to have extra dNTPs and magnesium so the reaction does not run out of components.
dNTPS
  • dNTPs are the nucleotide bases added to the growing DNA strand by the DNA polymerase.
MgCl2
  • The concentration of MgCl2 influences the stringency of the interaction between the primers and the template DNA.
Salt and buffers
  • Stabilize enzyme function and formation of primer template hybrids. Buffers often contain Tris HCl, KCl, and sometimes MgCl2.