Hemoglobin Electrophoresis Patterns in Sickle Cell Disorders (Acid)

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course . Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about (online CE course)
Hemoglobin Electrophoresis Patterns in Sickle Cell Disorders (Acid)

The following list corresponds to this image of an acid hemoglobin electrophoresis.

Lanes 1 and 2 contain controls ASC and AF (Remember, AF and ASC are labels and do not indicate the order of migration.)

Lanes 3 and 4 (patient 1): Heterozygous sickle cell trait (Hb SA) Hb S is approximately 30%

Lanes 5 and 6 (patient 2): Double heterozygous Hb SC disease (Hb SC)

Lanes 7 and 8 (patient 3): Homozygous sickle cell disease (Hb SS)
Hb S is 100%

Lanes 9 and 10 (patient 4): Sickle cell hemoglobin with hereditary persistence of fetal hemoglobin. Hb S is approximately 70%; Hb F 30%.