CAP Pro Course - Phlebotomy - Venipuncture (2026 & 2027)

(based on 26 customer ratings)

Author: Carol Becker, MS, MLS(ASCP)CM
Reviewer: Julie Ann West, PhD, MLS(ASCP)CM, SM(ASCP)CM and Joshua J. Cannon, MS, MLS(ASCP)CMSHCM

Continuing Education Credits

Objectives

  • Recognize the importance of proper patient preparation for venipuncture and describe these steps including patient approach, positive patient identification, and pre-collection requirements necessary for safe blood collection.
  • Identify blood collection tubes according to stopper color and additive and define the recommended order of draw sequence and its importance to specimen integrity.
  • Describe the steps for correct venipuncture performance, including site selection and preparation, and acknowledge common blood collection procedural errors.
  • Address appropriate post-venipuncture safety measures including needle safety, specimen labeling, and disposal of contaminated venipuncture supplies.
  • Explain potential patient complications and specimen integrity concerns resulting from venipuncture that compromises specimen quality and patient care.

Course Outline

  • Recognize the importance of proper patient preparation for venipuncture and describe these steps including patient approach, positive patient identification, and pre-collection requirements necessary for safe blood collection.
      • Introduction to Venipuncture
      • Patient Orders
      • Specimen Collection Priorities
      • Venipuncture Supplies
      • Approaching the Patient
      • Patient Care Partnership and Consent to Treatment
      • Patient Identification
      • Patient Preparation
      • Ashley is a phlebotomist who has arrived at a patient's hospital room to collect a fasting blood sample for cholesterol. She knocks on the door, then ...
      • The phlebotomist is notified to collect blood for a stat crossmatch on a hospitalized patient who requires immediate and unexpected surgery. Upon ente...
      • A phlebotomist is called to the emergency department (ED) to collect priority stat blood specimens from a trauma patient. The phlebotomist reports to ...
      • Handwashing is the single most important thing a phlebotomist can do in order to prevent the spread of infection from one patient to another. When sho...
      • Multiple orders for different tests with differing priorities are received in the laboratory for a hospitalized patient. The phlebotomist responsible ...
  • Identify blood collection tubes according to stopper color and additive and define the recommended order of draw sequence and its importance to specimen integrity.
      • Blood Collection Tube Basics: The Evacuated Tube System
      • Understanding Serum versus Plasma Requirements
      • Blood Collection Additives
      • The Order of Draw
      • Maintaining Specimen Integrity
      • Kate, the phlebotomist in the outpatient clinic today, receives an order for patient blood work for a test requiring serum. From the choices below, wh...
      • The quality of any laboratory result is crucially dependent on the collection of a quality specimen. A physician orders the following tests (note the ...
      • The order of draw in venipuncture is important for which of the following reasons?
      • A patient has a standing order for a CBC. The new phlebotomist selects a lavender-top tube for this draw. She notices, upon inspection of the tube, th...
      • Blood collection tube additives can have different functions. Which of the following is NOT one of these functions?
  • Describe the steps for correct venipuncture performance, including site selection and preparation, and acknowledge common blood collection procedural errors.
      • Proper Site Selection and Sites to Avoid
      • Cleansing the Site and Proper Tourniquet Application
      • Performing the Venipuncture
      • Common Venipuncture Procedural Errors
      • A test that requires a minimum of 2 mL of blood is requested on an adult patient. The phlebotomist is assessing a site for venipuncture and notices th...
      • Which antecubital vein should be considered as the last choice for use in venipuncture?
      • Which action should the phlebotomist take after cleansing the venipuncture site with 70% alcohol?
      • Which site listed is the best option for the phlebotomist to perform a routine venipuncture?
  • Address appropriate post-venipuncture safety measures including needle safety, specimen labeling, and disposal of contaminated venipuncture supplies.
      • Needle Safety Features and Disposal Methods
      • Specimen Labeling
      • Safe Disposal of Venipuncture Supplies
      • Alaina has completed her first-morning collection worklist and returned to the laboratory to accession and deliver the specimens to the appropriate de...
      • A young outpatient pulls their arm away as soon as the needle enters the vein during a venipuncture procedure. The phlebotomist acts quickly to stop t...
      • At what point in a routine venipuncture procedure should the blood tubes be labeled?
      • Which of the following is true with regard to venipuncture needles?
  • Explain potential patient complications and specimen integrity concerns resulting from venipuncture that compromises specimen quality and patient care.
      • Avoidance of Preanalytical Errors
      • Potential Patient Complications
      • Specimen Integrity Issues
      • A phlebotomist enters a patient's room with an order for a fasting blood glucose. The patient is just finishing breakfast. Which of these actions is m...
      • Ashley, a seasoned phlebotomist, has just met a patient who has a lot of blood work, priority stat. The patient's nurse has also just announced that t...
      • How should tubes be handled after successfully collecting the blood specimens?
      • What is the maximum time a tourniquet can remain on the arm before it is removed or released and then reapplied?
  • References
      • References

Additional Information

Level of Instruction: Basic
Intended Audience: This program is designed as an educational and training tool for phlebotomy, MLS, MT, and MLT personnel, medical laboratory science students and interns, pathology residents, and practicing pathologists.
Author: Carol Becker, MS, MLS(ASCP)CM is a retired Program Director for the Clinical Laboratory Science program and Histology program at OSF St. Francis Medical Center located in Peoria, Illinois. Carol received her Master's degree at the University of St. Francis in Joliet, Illinois and her Bachelor's degree in Medical Laboratory Science from the University of Illinois in Champaign, IL.
The author has no conflict of interest to disclose.  
Reviewer Information:
Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration Healthcare System, and has prior experience as an Administrative Laboratory Director.
Joshua J. Cannon, MS, MLS(ASCP)CMSHCM received his Bachelor of Science and Master of Science in Medical Laboratory Science from Thomas Jefferson University in Philadelphia, PA. He holds Medical Laboratory Scientist and Specialist in Hematology certifications through the ASCP Board of Certification. He was a professor at Thomas Jefferson University for seven years before transitioning into his current role as Education Developer at MediaLab by Vastian. His areas of expertise and professional passions include clinical hematology and interprofessional education. 

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