CAP Pro Course - Blood Bank - Antibody Screen and Identification

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Author: Suzanne H. Butch, MA, MLS(ASCP)CM, SBB, DLM Reviewers: Rory Huschka, M.Ed., MLS(ASCP) and Margaret Reinhart, MS, MLS(ASCP)

Continuing Education Credits

Objectives

  • Select problem-solving techniques based on serologic and clinical information.
  • Interpret antibody panels and apply rule-out strategies to identify antibodies.
  • Apply antibody characteristics to antibody identification.
  • Select additional testing procedures to confirm/rule-out presumed antibody identification.

Course Outline

  • Select problem-solving techniques based on serologic and clinical information.
      • Specimen Quality
      • Antibody Screening and Antibody Identification
      • Antiglobulin Reagents
      • Frequency of Antibody Identification
      • The First Steps in Problem Solving - Reviewing
      • Antibody Characteristics
      • Dosage
      • Selecting a Problem-Solving Strategy
      • Example of Selecting a Problem-Solving Strategy
      • The antibody screen below was performed on an automated instrument. What phenomenon is most likely being displayed? Rh-HrKellDuffyKiddLewisMNSsPLuther...
      • The following results were recorded for the antibody screen of a patient. When doing a patient history check, it was determined that the patient had b...
  • Interpret antibody panels and apply rule-out strategies to identify antibodies.
      • Antibody Identification Panels
      • Methods Used for the Initial Antibody Identification Panel
      • The Rule-Out Process
      • Cross-out Method
      • Rules for Rule-Outs
      • When the Antibody Cannot Be Identified in the Initial Panel
      • More About Probability of Rule-Outs
      • In the panel below, what are the most likely antibodies? Rh-HrKellDuffyKiddLewisMNSsP1LutheranXgDonor Cell NumberRhDCcEeCwKkKpaJsaFyaFybJkaJkbLeaLebMN...
      • Based on the results of the antibody screen for patient AF, the initial panel should be performed at what phases? Rh-HrKellDuffyKiddLewisMNSsPLutheran...
  • Apply antibody characteristics to antibody identification.
      • Patterns of Reactivity
      • Strength of Reactivity
      • Phases of Reactivity
      • In the panel below, what additional testing should be done before the antibody can be concluded? Rh-HrKellDuffyKiddLewisMNSsP1LutheranXgDonor Cell Num...
      • The antibody screen and panel were performed using automated solid phase technology. Which of the following testing methods would be most helpful for ...
  • Select additional testing procedures to confirm/ruleout presumed antibody identification.
      • Confirming the Antibody
      • Selection of Additional Cells
      • Antigen Typing
      • Enhancement and Selection Methods
      • Enhancement and Selection Methods, continued
      • Adsorptions and Elutions
      • Putting It All Together
      • Patient GS has the panel results below. What is the most logical next step in resolving this problem? Rh-HrKellDuffyKiddLewisMNSsP1LutheranXgDonor Cel...
      • Selective adsorption and elution is a technique used to accomplish which of the following?
  • References
      • References

Additional Information

Level of Instruction:  Intermediate
 
Intended Audience:   This program is designed as an educational and training tool for MLS, MT, and MLT personnel, medical laboratory science students and interns, pathology residents, and practicing pathologists.
 
Author Information:   Suzanne H. Butch, MA, MLS(ASCP)<sup>CM</sup>, SBB, DLM is currently working on special projects for the Department of Pathology at Michigan Medicine in Ann Arbor, Michigan. She formerly worked in Quality Assurance in the Department of Pathology and as the Administrative Manager for Healthcare, Blood Bank & Transfusion Service at the University of Michigan Hospitals and Health Centers in Ann Arbor, Michigan. She holds a Bachelors in Medical Technology from the University of Michigan, a Masters's Degree in Management and Supervision from Central Michigan University, and Certifications as a Specialist in Blood Bank, as a Quality Audit and as a Diplomate in Laboratory Medicine.
 
Reviewer Information: Rory Huschka, M.Ed., MT(ASCP), has over 20 years of experience as a medical technologist, technical supervisor, professor, and manager. He is the former Director of Medical Laboratory Science and Medical Laboratory Technician Programs at Brookline College. He is currently a Program Director at MediaLab, Inc. Rory holds a BS degree in Medical Technology from North Dakota State University and a Master's Degree in Educational Leadership.  
 
Reviewer Information:  Margaret Reinhart, MS, MT(ASCP) is a Senior lecturer in Biological Sciences at the University of the Sciences in Philadelphia, PA where she teaches hematology, clinical immunology, parasitology, and other related courses. She was the MLS Program Director there for 30 years. She is also an adjunct instructor in Hematology at Pennsylvania Hospital, Philadelphia, PA. She holds a Master's Degree in Biology and in Health Care Administration.
 
 
 
 

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