CAP Pro Course - Histology - Special Stains

Continuing Education Credits

• P.A.C.E.® Contact Hours: 1 hour(s)
• Florida Board of Clinical Laboratory Science CE Credit Hours -
  General (Histology): 1 hour(s)

Objectives

• Identify common staining methods that are routinely employed in the histology laboratory setting and discuss the clinical/diagnostic purpose for performing special stains.
• Discuss processing considerations, including appropriate fixative based on special stain procedure, function of mordants, and microtomy.
• Determine the use of appropriate controls as a quality assurance measure.
• Discuss requirements for accurate dilution of special stains, including solution labeling, storage, safe-handling, and disposal.
• Recognize staining errors and discuss troubleshooting considerations.

Course Outline

• Identify common staining methods that are routinely employed in the histology laboratory setting and discuss the clinical/diagnostic purpose for performing special stains.
• Clinical Necessity of Special Stains
• Common Staining Methods for Demonstration of Amyloid and Carbohydrates
• Common Staining Methods for Demonstration of Connective Tissue, Muscle Fibers, and Lipids
• Common Staining Methods for Demonstration of Microorganisms
• Common Methods for Demonstration of Minerals
• Common Staining Methods for Demonstration of Nervous System Components
• Both the Gomori's one-step trichrome stain and Masson's trichrome stain are used to distinguish collagen from muscle tissue. While both of these stain...
• You are performing a Jones' methenamine silver stain on a kidney biopsy section. In reviewing the staining procedure, you take note of the gold chlori...
• Dr. McTavish is reviewing a liver biopsy case in which glycogen storage disease is suspected. As such, he requests a periodic acid-Schiff (PAS) stain....
• Discuss processing considerations, including appropriate fixative based on special stain procedure, function of mordants, and microtomy.
• Processing Considerations and Appropriate Fixative for Lipid Demonstration
• How Do Mordants Work?
• Secondary Fixation: Mordant Precedes the Dye
• Use of Mordant-Dye Complex in Single Staining Solution
• Mordant Use Following Dye
• Appropriate Fixative Selection Based on Special Stain Procedure
• Microtomy Considerations for Special Stains
• In the Verhoff-Van Gieson stain for elastic fibers, what is the function of ferric chloride in the Verhoff solution?
• Which of the following is the MOST appropriate fixative for frozen (cryostat) sections intended for oil red O staining?
• Alex is performing a Gomori's one-step trichrome stain on a liver biopsy. Which of the following best describes the chemical action of Bouin's fluid w...
• Determine the use of appropriate controls as a quality assurance measure.
• Quality Control (QC) of Special Stains
• Use and Evaluation of Special Stain Control Sections
• Appropriate Controls for Amyloid and Carbohydrate Staining Methods
• Appropriate Controls for Connective Tissue, Muscle Fiber, and Lipid Staining Methods
• Appropriate Controls for Mineral Demonstration
• Appropriate Controls for Microorganism Staining Methods
• Appropriate Controls for Nervous System Component Staining Methods
• Quality control pertaining to special stains involves many factors, including clean glassware. Which of the following describes the MOST appropriate g...
• Tess is reviewing several special stain procedures and needs to include verbiage specific to positive tissue controls. Which of the following statemen...
• In reviewing the inventory of special stain positive control blocks, you observe several blocks labeled as "normal cerebral cortex." Which of the foll...
• Discuss requirements for accurate dilution of special stains, including solution labeling, storage, safe-handling, and disposal.
• Storage and Labeling Requirements for Special Stain Solutions
• Safe Handling and Disposal of Special Stain Solutions
• Creating Special Stain Solutions with the Use of Dry Powder Chemicals
• Creating Special Stain Solutions of Accurate Dilution
• When pouring staining solutions from a primary container into a secondary container, which of the following is a TRUE statement with regard to the sec...
• The Jones' methenamine silver requires 3% sodium thiosulfate. If 100 mL of this solution is created, how much sodium thiosulfate is required for an ac...
• You have been asked to decolorize a slide that was previously stained with hematoxylin & eosin. To do this, you will need to make up an 80% alcoho...
• You have found a container of staining solution that is expired. The container label indicates that the solution is flammable. Which of the following ...
• Recognize staining errors and discuss troubleshooting considerations.
• Avoiding Problems with Staining Procedures and Staining Reagents
• Troubleshooting Special Stains for Amyloid and Carbohydrates
• Troubleshooting Special Stains for Connective Tissues, Muscle Fibers, and Lipids
• Troubleshooting Methods of Mineral Demonstration
• Troubleshooting Special Stains for Microorganisms
• Troubleshooting Special Stains for Nervous System Components
• Which of the following is the MOST likely cause of weak methylene blue counterstaining results in the Ziehl-Neelsen AFB stain?
• In the Luxol Fast Blue (LFB) stain for myelin sheath, what processing step is required for accurate staining of myelin?
• References
• References

Additional Information