CAP Pro Course - Blood Bank - Direct Antiglobulin Test

Continuing Education Credits

• P.A.C.E.® Contact Hours: 1 hour(s)
• Florida Board of Clinical Laboratory Science CE Credit Hours -
  General (Blood Banking / Immunohematology): 1 hour(s)

Objectives

• Discuss the basic principles of antiglobulin testing and clinical indications for direct antiglobulin testing (DAT).
• Describe serological procedures used in DAT testing and the preanalytical and analytical variables that may influence results.
• Identify clinical conditions that may cause positive DAT results.
• Describe common methods used in the investigation of a positive DAT.

Course Outline

• Discuss the basic principles of antiglobulin testing and clinical indications for direct antiglobulin testing (DAT).
• Antiglobulin Testing Principles
• Antibody-Coated Control Cells
• Clinical Indications for Direct Antiglobulin Testing
• Anti-human Globulin Reagents
• The DAT is used to detect the presence of antibodies coating the red cells in which one of the following diseases?
• Which one of the following reagents should be selected to be used as a screening test for detecting red cell sensitization?
• In which one of the following situations is a DAT required?
• Which one of the following might result in false-positive DAT results?
• Describe serological procedures used in DAT testing and the preanalytical and analytical variables that may influence results.
• DAT Specimen Requirements
• DAT Serological Procedures
• Which one of the following is the best specimen to use for direct antiglobulin testing?
• A technologist is performing a DAT using anti-complement reagents. The reagent is added to the washed cells and centrifuged. The test reaction is read...
• When should a patient's cells be washed with 37°C saline prior to performing a DAT?
• Prior to surgery, a pre-transfusion workup on patient F resulted in an ABO discrepancy. Records show that patient F has a history of cold agglutinin s...
• Identify clinical conditions that may cause positive DAT results and variables that may cause false-positive and false-negative results.
• Interpretation of Positive DAT Results
• Autoimmune Hemolytic Anemia (AIHA)
• Hemolytic Transfusion Reactions (HTRs)
• Drug-Induced Hemolysis
• Hemolytic Disease of the Fetus and Newborn (HDFN)
• Preanalytical and Analytical Variables That May Produce False-Positive DAT Results
• Preanalytical and Analytical Variables That May Produce False-Negative DAT Results
• A technologist reads a panel made from the eluate of a patient with warm autoimmune hemolytic anemia (WAIHA). There is reactivity with all cells, but ...
• A patient has been taking a high dose of a medication known to cause hemolysis. What is the first step in determining if the patient's anemia is cause...
• Describe common methods used in the investigation of a positive DAT.
• Evaluation of Positive DAT Results
• Elution Techniques
• Adsorption Techniques
• Evaluation of Positive DATs in Newborns
• What cause of a positive DAT is most likely to produce pan-agglutination in the AHG phase of an antibody screen?
• Patient W is a 47-year-old male post-operative patient whose hemoglobin levels have been decreasing. He received two units of Red Blood Cells during s...
• Patient R is being evaluated for a suspected delayed hemolytic transfusion reaction. The post-transfusion serological work-up is summarized below. Wha...
• Patient T is a 58-year-old woman with a history of warm-autoimmune hemolytic anemia. Her physician has ordered two units of Red Blood Cells to be read...
• References
• References

Additional Information