Technical Competence in Paraffin-Based Fluorescence In Situ Hybridization (FISH)

Continuing Education Credits

• P.A.C.E.® Contact Hours: 1.5 hour(s)
• Florida Board of Clinical Laboratory Science CE Credit Hours -
  General (Histology): 1.5 hour(s)

Objectives

• Describe the theoretical concepts used in fluorescence in situ hybridization (FISH) testing.
• Demonstrate an understanding of the methodologies involved in FISH testing.
• Explain the enumeration of fluorescence signals in paraffin-based FISH testing.
• Identify the equipment necessary to perform FISH testing in a clinical laboratory.

Course Outline

• Importance of Fluorescence In Situ Hybridization (FISH) Testing in Cancer Care
• Detecting Genetic Aberrations with Fluorescence In Situ Hybridization (FISH)
• Drug Therapy Based on Genetic Aberrations
• DNA Content and Ploidy
• Genetic Aberrations
• Traditional Cytogenetics
• Metaphase Fluorescence In Situ Hybridization (FISH)
• Interphase Fluorescence In Situ Hybridization (FISH) in Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue
• What type of fluorescence in situ hybridization (FISH) is performed on formalin-fixed, paraffin-embedded (FFPE) tissue?
• Theoretical Knowledge Required for Fluorescence In Situ Hybridization (FISH) Testing
• Chromosomes
• Centromeres
• Probes
• Centromere Probes
• Locus-Specific Probes
• Break-Apart or Translocation Probes
• Which of the following probe types are used to detect a translocation?
• Equipment used in FISH technology
• Equipment Required to Prepare and Store Reagents
• Equipment Required for Preparation of Tissue
• Equipment Required for Manual Performance of Fluorescence In Situ Hybridization (FISH) Assay
• Equipment Required for Analysis of Fluorescence In Situ Hybridization (FISH) Slides
• Photographing Fluorescence In Situ Hybridization (FISH) Slides
• All of the following are REQUIRED in order to perform a fluorescence in situ hybridization (FISH) assay EXCEPT:
• FISH methodology
• Tissue Preparation
• Common Methodologies
• Slide Preparation
• Pre-treatment Strategies
• Standard Pre-treatment
• Standard Protease Digestion
• Standard Denaturation
• Standard Probe Preparation and Hybridization
• Standard Post-Hybridization and Completion
• New Histologic-Based Fluorescence In Situ Hybridization (FISH) Technique
• Differences in Pre-treatment
• Differences in Digestion
• Which of the following pre-treatment solutions are more harsh to the tissue?
• It is acceptable to use the digital reading on the water bath during the FISH assay.
• It is important to denature the tissue DNA and the probe DNA.
• What happens during the hybridization step of a FISH assay?
• Enumeration of FISH signals
• Assessing Slide Quality
• Control Slide
• Patient Slide
• HER2/neu Examples
• Reporting Fluorescence In Situ Hybridization (FISH)
• International Standing Committee on Cytogenetic Nomenclature (ISCN) Reporting
• A final interpretation of FISH results is required in the report.
• The following image is of a break apart probe for ALK translocation. Is the image depicting a normal or an abnormal sample?
• References
• References

Additional Information